Methods

Neuroanatomy

We study the effects of chronic stress and dieting on the morphology of neurons.

Pyramidal neuron of the prefrontal cortex and its 3D reconstruction.

Methods

We combine in situ hybridization with immunohistochemistry. In situ hybridization is used to detect mRNA, and immunohistochemistry to detect protein. 

CRF mRNA (silver grains) and Fos protein (brown staining)

Methods

We use double-immunohistochemistry to detect what kind of neurons is activated by expressing Fos protein, a molecular marker of persistent neuronal activation.

This example shows that all the vasopressing neurons (brown stating) are activated and express Fos protein (black staining)

Methods

This example clearly shows that oxytocin neurons (brown staining) do not activate expression Fos protein.

Methods

We study the neuronal connections within the brain using the retrograde and anterograde tracing.

When we want to know the brain region where our brain structure of interest projects, we use an anterograde tracer. This example demonstrates the injection site of the anterograde tracer biotinylated dextran amine into the nucleus of the solitary tract (NTS) and the axonal projections from the NTS (shown by the black arrows)

Methods